>Dear Flowers, >I always work with Cell Quest but now I downloaded Flow Jo program >and I have a problem. >I don't know where is the option for create the union of two gates ( >In Cell Quest is R1 or R2) >Then I would to know wich are the substantial differences between >Cell Quest and Flow Jo >Thanks in advance >Ciao >Simona FlowJo uses a hierarchical rather than a logical gate structure, ie you don't work by creating two gates and then combining them, rather you place the first gate, open the gated plot and place the second gate directly on that plot. The best way to get into FlowJo is to do the tutorial that can be downloaded from the web-site (<http://www.flowjo.com/tutorial.html>). Until you have got your head around the fundamental differences between the programs it is very hard to compare them. Adrian
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