Yes. We detect alloantibody on the surface of human leukocytes in organ transplantation routinely. Jackson Immunolabs sells Fab2 anti-human Ig(H+L) for IgM and IgG conjugated to various fluorochromes. You will want to co-stain with anti-CD3 to exclude staining of B cells, which can be problematic (express FcR, and surface Ig) Martin S. Zand, MD, PhD Assistant Professor of Medicine and Medical Humanities Medical Director, Kidney and Pancreas Transplant Programs University of Rochester Medical Center 601 Elmwood Ave - Box 675 Rochester, NY 14642 Vox: 585.275.4517 Fax: 585.442.9201 -----Original Message----- From: Fang-yao Stephen HOU [mailto:shou@uci.edu] Sent: Saturday, March 02, 2002 5:24 AM To: cyto-inbox Subject: detecting autoantiboy on cell surface (human) Dear experts in clinical cytometry: One of my users asks me if it is feasible to detect human IgM/IgG on surface of leukocytes. The white count of the patient is between 30k - 40k. The oncologist is looking for venders with supply of anti-human IgM & IgG, conjugated with fluorochromes. A related literature regarding the detection of surface bound autoantibody will be very helpful as well. Thank you very much. Fang-Yao Stephen Hou Flow Cytometry Specialist UC-Irvine
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