Hello For those of you who know the true finer details about flow and fluid dynamics ... can somebody tell me the calculation to work out the volume size of droplets. The mathamatical approach rather than collecting and counting the volume. We are using a 50 µm nozzle at 30psi (BD vantage) and trying to single cell sort bacteria onto 96 well plate. The first question is how large are the droplets (volume) ? Can you determine the dilution factor of your samples (bacteria) ? We are sorting bacteria and triggering on the fluorescence signal to reduce background noise. The questions is : at what rate of sample can we assume that the instrument is detecting a single cell in a droplet ? Looking at the numbers it would appear that non-fluorescent bacteria must be in the same droplets, but I may be assuming something incorrectly. Any help appreciated and further information available if it will help you with the solution ! Andy
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