SP with LSR

From: David Dombkowski (dombkows@helix.mgh.harvard.edu)
Date: Mon Mar 04 2002 - 08:32:45 EST

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Kimmo,

    You will not see red fluorescence with Hoechst 33342 with the
configuration of 2 PMT UV, 3 or 4 PMT Argon and 1 PMT He-Ne. The red
fluorescence is too high for dichroic to reflect to PMT for UV. You need to
be able to collect this signal in PMT usually dedicated to Argon or He-Ne
laser. There is no pinhole for UV fluorescence in these channels. You need
a custon configuration which may be achieved if you cantact Becton
Dickinson.

David

David M. Dombkowski
dombkowski@helix.mgh.harvard.edu
Flow Cytometry-Pathology-CNY rm7017
149 13th Street
Massachusetts General Hospital-East
Charlestown, MA 02129
Tel. (617)726-1683
Fax.(617)724-3164

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