I have a client who wish to run flow on nematode eggs (Meloidigyne incognita and Meloidigyne hapla) to stain to look at genome size. We have tried twice to run them and the first time saw no florescence so we change protocols to PI but the second time we clogged both my machines with the samples( I am using a BD FACScan and a FACSCalibur). Can anyone help us-she has found a paper from 1984 which used flow on a machine made at Los Alamos but it doesn't specify what size nozzle they used. She also does not know how big the eggs are but you can visualize them in the solution and they settle out of solution very quickly. thank you in advance for all your help-I have always had such great luck with questions on this group. Sincerely Janet Dow Janet Dow Research Technician and Manager Flow Cytometry Facility North Carolina State College of Veterinary Medicine Room C-314 Raleigh, NC 27606 (919)513-6364
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