Dear Flowers: I would like to seek your expertise on stem cell analysis. Here is my question. We have a user who purified the erythrocytic stem cells by magnetic beads. These preliminarily purified cells without any flourescent staining showed two cell populations at FL3 (at negative area and positive area, respectively). At the rest of PMTs, they all were located at negative range. Has anyone had similar experience before? Please advise. Thank you very much. Best regards, Yen-Chi Chen BD Taiwan, BDB, COE Tel: 02-2722-5660 ext 297 Fax: 02-2725-1768 yenchi_chen@bd.com
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