thanks to everyone for responding to my question about my gamma delta t cell staining. I have just one more question before the weekend starts. We have a problem with how we can tell whether some events we are looking at in our flow data are single events or actually doublets appearing as a single event. The reason is because we are looking at a cell population that appears to increase in size upon activation (not an all too uncommon event) that appears to express to phenotypic markers. Our question was simply are we seeing one single eukaryotic cell expressing our 2 markers of interest or actually 2 separate cells stuck together each expressing it's own marker. I thought that maybe the best way to approach this was to repeat some version of the experiment and perform a DNA ploidy test, or something along those lines. thoughts? I hope I have given enough detail. thanks, -- Wayne Harris Lead Research Specialist Department of Microbiology and Immunology Emory University , School of Medicine
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