Hi Francesca... Actinomycin D can be prepared as a 1 - 10 mg/ml stock in 95% ethanol and stored at -20C. It will remain active for at least a year. Cycloheximide is water-soluble and can be dissolved directly in tissue culture media if desired. We usually make our cycloheximide stocks fresh, I don't know how stable they are over time (although the dry form can last for years). Bill Telford NCI-NIH At 04:08 PM 2/21/2002 +0100, Francesca Mazzotti wrote: >Hi! >I have a simple question. >I know that protein transcription or translation inhibitors such as >actinomycin D or cycloheximide induce apoptosis in murine fibroblasts. >Therefore they could make a useful positive control. >May I know in which kind of solvent (water, ethanol,...) I have to dissolve >these substances? > >Thanks, > >Francesca Mazzotti
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