plant protoplast sorting

From: Gary A. Ward (Gary.A.Ward@dartmouth.edu)
Date: Thu Feb 21 2002 - 11:21:21 EST


Hello Flowers,

I'm gearing up to do some plant protoplast sorting and wondered what kind of experience
or advise people on the net might have to offer?
I looked up where there was some discussion last summer but most of it had to do
with dyes.  There were a couple of questions that may have been answered privately
because the answers weren't posted.
Any suggestions about a specific nozzle size or buffer that would make these cells
viable after sorting?  If anyone out there has suggestions or advice would you please
email or call.

Thank  You!

Gary A. Ward
H C Englert Cell Analysis Laboratory
Norris Cotton Cancer Center
Lebanon, NH USA

603/650-7907=20

gary.ward@dartmouth.edu



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