Dear Calman, We, at CLB, have outstanding monoclonal and polyclonal a-IgG4 antibodies. Monoclonal mouse-anti-human antibodies are both unlabeled and HRP labeled available, product code M1271, anti-human IgG4 (CH3), clone MH164-4, HP 6196 (unlabeled) and M1331 (labeled). Regards, P. Stam John Voorn CLB Reagents Productmanager Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl> -----Original Message----- From: Calman Prussin [SMTP:CPRUSSIN@niaid.nih.gov] Sent: Tuesday, February 12, 2002 3:50 PM To: Cytometry Mailing List Subject: Abs to human IgG4- which? Dear flowees- I am trying something new, an immunoassay that requires an Ab to human IgG4. Since I usually use mouse Abs (and anti-mouse Ig secondaries) I am totally ignorant of who supplies the best antibodies to human IgG. My favorite source for goat anti-mouse polyclonals (SBA) does not carry goat anti-human IgG4. Questions: 1. Does anyone know of a great high signal-to-noise mAb or pAb to human IgG4 conjugated with biotin? to a phycobiliprotein? 2. PharMingen has 2 mAb clones, each of which recognizes a different epitope. I could use them both and increase my S/N. Any experience with these? Thanks, Calman _______________________ Calman Prussin Laboratory of Allergic Diseases NIAID/ National Institutes of Health
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