RE: [HIV Back-titration?]

From: Nicholson, Janet (jkn1@CDC.GOV)
Date: Wed Feb 20 2002 - 15:11:57 EST

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Comment from a colleague:

Back titration is always done when assaying for optimal infectious units of
virus.  TCID50's or TCID90's is often the prefered unit when calculating for
most viral inhibitory activities.

-----Original Message-----
From: André G. Loxton [mailto:GL2@sun.ac.za]
Sent: Wednesday, February 20, 2002 1:26 AM
To: cyto-inbox
Subject: [HIV Back-titration?]

 Hi,

 can anyone out there that does HIV research tell me whether its
standard procedure to do back-titrations of the HI-virus.

 I know you perform this for other viruses, but have never come
across any such info for HIV.

If possible, can you refer me to some articles?

 Thanx

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