plate coating with anti-CD40

From: Karim Vermaelen (Karim.Vermaelen@rug.ac.be)
Date: Mon Feb 18 2002 - 04:10:07 EST


Hello,
I realize this question is a little bit off-the-flow, but can anyone
recommend a sensible protocol for immobilizing anti-CD40 Ab (clone 3/23,
BD-Pharmingen) to 96 well or 24 well plates? There seems to be quite
diverging recipes in terms of incubation time, Ab concentration and buffer
composition...
Thanks for any suggestions/references!
Karim



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