Dear Purdue site: Currently we use a 1:4 dilution of methanol free, EM grade, 10% formalin in PBS as a fixative for immunophenotyping of human blood. We have some preliminary evidence that this concentration is contributing to a decrease in fluorescence for CD45RA Fitc when analyzed at 24 hours. A literature review indicates this is a stronger concentration than the norm (0.5, 1.0 or 2%). Our specimens are processed with either FACS Lysis or Coulter Immunoprep, then fixed. Any opinions or suggestions concerning what concentration best preserves as well as inactivates viral components? Thanks. Lorrie Epling Laboratory Supervisor UCSF/SFGH Core Immunology Lab General Clinical Research Center Phone 415-476-5279 Fax 415-206-8200
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