Hi Ruth, Not answering the question but have you tried using Annexin Cy3 in place of PE? I found that in certain cell types, annexin-FITC gives nice results while annexin-PE shows nothing - I suspect it may be a steric affect of using the large PE molecule as replacing with annexin-Cy3 gives results comparable to the FITC. Although Cy3 isnt optimally excited at 488nm, there is sufficient signal for it to be detected - the emission maximum is about 570nm. I get annexin-Cy3 from MBL: http://www.mblintl.com/products/annexinkit.html Good luck! Derek On Fri, 8 Feb 2002 Ruth_Wilkins@hc-sc.gc.ca wrote: > Does anyone know of a human antibody for neutrophils that is available in any > colour besides FITC? I am trying to measure Annexin labelling in neutrophils > and am having problems with Annexin-PE but Annexin-FITC works well. ************************************************************************ Derek Davies Voice: (44) 020 7269 3394 FACS Laboratory, FAX: (44) 020 7269 3100 Cancer Research UK, e_mail: derek.davies@cancer.org.uk London, UK mobile: 07790 604112 Web Page: http://sci.cancerresearchuk.org/axp/facs/davies/index.html In tenebris lux *************************************************************************
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:59:24 EST