Dear colleagues, I would like some ideas on how to differentiate between two situations by flow cytometry, decreased expression of an antigen due to margination of high expressors or due to downregulation. Here is the scenario. Approximately 80-90% of monocytes expressed marker X normally. However with disease this may decrease to 60-70%. Since there is a population of negative cells already, the proportion of these cells will increase if there is margination or sequestration of high expressors or if there is downregulation leading to an increase in the number of negative cells. How do I distinguish between these two possibilities? Any assistance will be greatly appreciated. Regards, Jose -- José A. Stoute, MD Unit 64109, Box 401 USAMRU-Kenya APO AE 09831-4109 e-mail: jstoute@kisian.mimcom.net Nairobi Tel 254-2-729303, Fax 254-2-714592 Kisumu Tel 254-35-22942, Fax 254-35-22903 Electronic Fax Service: 1-630-214-2008
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