Hello Vivian I would refer you to the following references; DNA AND CELL CYCLE ANALYSIS AS PROGNOSTIC INDICATORS IN BREST TUMORS REVISITED, by C. Bruce Bagwell MD, PhD, Gary M. Clark, PhD, Frederique Spyrantos, DrSc; et al: in New Applications of Flow Cytometry, pp 875-895, vol 21 Number 4, December 2001, Published by Clinics in Laboratory Medicine Optimizing Flow Cytometric DNA Ploidy and S-Phase Fraction as Independent Prognostic Markers for Node-Negative Breast Cancer Specimens; by C. Bruce Bagwell MD, PhD, Gary M. Clark, PhD, Frederique Spyrantos, DrSc; et al: in Cytometry (Communications in Clinical Cytometry) 46:121-135 (2001) Published by Wiley-Liss, Inc. Adjusting to the new role of DNA Analysis in Breast Cancer Prognosis, Yuan CM*, Clevenger C, McCarthy T, et.al.: Poster Presented at the Clinical Cytometry Society’s 16th Annual Clinical Applications of Cytometry Symposium, Coronado Springs Resort, Orlando, FL. November 11-13, 2001 *yuanc@uphs.upenn.edu, University of Pennsylvania School of Medicine, 297 John Morgan Bldg., Philadelphia, PA Best regards Don Donald J. Herbert Technical Support Manager Verity Software House, Inc. PO Box 247 45A Augusta Road Topsham, ME, USA 04086 Phone: (207) 729-6767 ext.190 Fax: (207) 729-5443 email: tech@vsh.com web: www.vsh.com -----Original Message----- From: Vivian Wu [mailto:ffwu@telus.net] Sent: Thursday, February 07, 2002 12:53 AM To: Cytometry Mailing List Subject: Basic S-Phase Question Dear Fellow "Flowers" As a neophyte into the realm of DNA Flow, can someone please point me in the direction of either text or article regarding the ABCs of establishing the S-Phase Cutoffs? Our interest is in proliferating non-malignant lymphocytes. Modfit LT will be used in the analysis. Vivian Wu Immunology Lab Vancouver General Hospital email: ffwu@telus.net
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