[Fwd: Paul Jolicoeur]

From: Nathalie Tessier (tessien@ircm.qc.ca)
Date: Fri Feb 08 2002 - 08:52:00 EST


Good morning everybody,

One of our user is having problem with staining. kupffer cells. Does anybody
has experience in this.

Thank you

Nathalie Tessier

Rita Gingras wrote:

> Message du Dr. Paul Jolicoeur à :
>
> Nathalie Tessier
>
> Dear Nathalie,
>  We are having problems labeling the liver Kupffer cells (macrophages)
> with different antibodies by FACS.  The isotype controls label the cells
> as intensely as the antibodies .  As you know, these cells are designed
> to actively phagocytose.  Is there a trick to get specific labeling
> easily?
>
> Sincerely,
>
> Paul, Jolicoeur, M.D., Ph.D.
> Directeur
> Laboratoire de Biologie Moléculaire
> Téléphone :  (514) 987-5569
> Fax  :  (514) 987-5794
> Courriel:  jolicop@ircm.qc.ca

--
Nathalie Tessier, M.Sc.

Adjointe au directeur de l'administration de la recherche
Responsable, Service de cytométrie en flux

Assistant director, Administration of research
Head of flow cytometry service

Institut de Recherches Cliniques de Montréal
110, ouest avenue des Pins
Montréal, Québec, Canada
H1R 1N7
tel:(514) 987-5608
fax:(514) 987-5724



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