Re: FDA Regulation and cytometry

From: Betsy M. Ohlsson-Wilhelm, Ph.D. (bmow@scigro.com)
Date: Wed Jan 23 2002 - 11:18:17 EST


Hi Lea,

I passed your query on to my partner’s husband, Dr. Alan Muirhead, who is
involved in certain types of validations. Here is his reply:

Betsy,

(somehow the left side of the message was cut off, but I think the intent is
clear; if you see something in the original that would be important to know,
let me know)

Typically, the manufacturer is the only one who would offer to supply a
"validation package" for an instrument. That package would include
Installation Qualification (IQ) and Operational Qualification (OQ) test
scripts. IQ usually is how do you install the system--often checklists of
hook this to that, load these files off of that CD, etc, plus confirmations
of the environment as  appropriate (40 amp supply at 220 volts, 20 tons of
air conditioning, ...). OQ is a minimal verification that the system works:
run a standard sample, get an expected histogram.

Unless some firm has defined a niche of validating these instruments, (and I
don't know that end of the market), I wouldn't expect anyone else to offer a
canned IQ/OQ package.

What the writer is missing is the rest of what is meant by validation. First
of all, a canned IQ/OQ package does not provide any sort of end user test
(also called performance qualification, or PQ). At the very least, the PQ
would have to include method verification for how the user intends to use
the instrument. The manufacturer can't do that.

Second, the FDA has made it clear that validation begins with a validation
plan and a set of end user specifications, before any kind of IQ/OQ/PQ can
begin. Producing these formal documents--especially user specs--has not been
the usual practice in the lab world, but the Part 11 regulation and the new
Guidance on Validation do not make exceptions for labs. The agency's
position is that the end user testing must demonstrate that the user
requirements have been met, and you can't do that without a requirements
spec.

Finally, Part 11 compliance depends at least as much on procedures and
policies in force at the user site as it does on the technical capabilities
of the system. So no one can say if FACSCalibur would be compliant without
seeing how it was implemented and used. At best, the manufacturer could say
that FACSCalibur has the technical features that would allow a Part 11
compliant installation (e.g., audit trails, secure logins, no deletion of
records, electronic signature capabilities). But then it's still up to the
purchasing company to be sure that all users have unique IDs, that passwords
aren't shared, that the file systems are secure, etc.

Hope this helps,
Alan

If you wish to contact Alan directly, his email is muirhead@voicenet.com
<mailto:muirhead@voicenet.com>	.

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Betsy M. Ohlsson-Wilhelm, Ph.D., CEO
SciGro, Inc./NorthEast Office
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E-mail: bmow@scigro.com
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