Hello Cytometrists, I was reading the Article "Polarization of Scatter and Fluorescencs Signals in Flow Cytometry" (C.L Asbury, J. L. Uy and G. van den Engh: Cytometry 40/ 2 p88-101) Amongst other in the paper they discribe why two particles that emit the exactly same amount of fluorescence, but with different degrees of anisotropy will not emit with the same intensity towards the detector. I must admit that I did not understand every detail but I thought I more ore less understood what they were talking about. Except for this statement in the discussion: " Althought one migth expect otherwise, this artifact [different intensity measured from two particles with different anisotropy and same fluorescence] cannot be avoided by using a circularly polarized laser or a light beam from an unpolarized lamp. Whereas using a circualrly or randomly polarized excitation beam will reduce the degree of fluorescence anisotropy, it will not completely eliminate it." Unfortunately here they stop with their explanations and thought I tried to find out by my own why this must be so (Gedankenexperiment :-) ), I seem to be too stupid to suceed. So I thought maybe someone else in this list knows and could enlight me. Thanks in advance Greetings Claudio =================================================== Claudio Vallan PhD Phone Lab: 031 / 632 88 76 FACS-LAB DKF Phone Office: 031 / 632 99 68 University of Bern E-Mail: vallan@dkf7.unibe.ch c/o Institute of Pathology Murtenstrasse 31 Insel hosptial area only: 3010 Bern Beeper: 181 67 59 Switzerland ===================================================
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