SNARF®-1 carboxylic acid, acetate, succinimidyl ester http://www.probes.com/servlets/product?region=Select&item=22801 is a very new probe that we have not yet tested for assessing cell cycle. We have tested it for labeling cells and for cell tracing. I am not aware of any publications that have used it for that, however. However, it requires the same hydrolysis of the acetates as does CFSE and has the same succinimidyl ester as CFSE and should therefore have similar utility and have its fluorescence decrease by half on cell division, as does CFSE. Its potential advantage is that it can be excited at 488 nm but has red fluorescence so it may be complementary to CFSE.
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