Kevyn, I went away from the Clenz (the blue stuff) because of precipitates that formed if the instrument sat for more than a couple days ie,holidays, they were difficult to clean out of the fluidics. I personally use a 50/50 mixture of prediluted "hemoturge" (the green stuff) and 70%EtOH. VWR #45001-014 in a 4Liter box with spout. Advertized as a hematology detergent, azide free for cleaning Coulter Counter apetures and tubing. This seems to work well for 90% of regular cell work. However when some particularly sticky stuff is passaged through the XL such as cultured keratinocytes or the contents of a mouse ear pouch prep that is a nasty mixture of inflammatory cells and yeast membranes we go with a commercial product, good old 'Windex'. First introduced to me years ago by Alton Floyd Ph.D. He had parked a Facs 440 with it a year previous to us inheriting the machine. When we hooked it up to pressure without doing anything else we had flow!. Since then many others have lauded its utility. Don't advertise that you also can do windows. One last suggestion, given to me by the alternative service company that takes care of our XL, is a product called 'Jet Dry' Its on the market, I think, as a liquid automatic dish washer fluid. The service guy who has some 20 years experience working with hematology and flow instruments said that when all else fails he blows some of this through and it hasn't failed him yet. Since its in his best interest not to self generate future calls I feel good about using it. Good Luck Don Walker Celltech R&D Inc. Seattle -----Original Message----- From: Kevyn Adler [mailto:kadler@miltenyibiotec.com] Sent: Friday, January 11, 2002 2:50 PM To: cyto-inbox Subject: Composition of Coulter Clenz Flow Cytometrists, I have a couple of questions regarding protein, lipid, and cellular debris removal. Does anyone know the ingredients for Coulter Clenz and FACSClean? Which one would be better for a system that has different type of tissues (spleen, lymph node, bone marrow) and cell types pushed throught it? Is there a simple solution (trypsin or SDS wash) that could by used to remove these contaminates without hurting a teflon tubing set? Thank You, The information contained in this email is intended for the personal and confidential use of the addressee only. It may also be privileged information. If you are not the intended recipient then you are hereby notified that you have received this document in error and that any review, distribution or copying of this document is strictly prohibited. If you have received this communication in error, please notify Celltech Group immediately on: +44 (0)1753 534655, or email 'is@celltech.co.uk' Celltech Group plc 216 Bath Road, Slough, SL1 4EN, Berkshire, UK Registered Office as above. Registered in England No. 2159282
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