FISH and flow

From: Cheryl Smith (cheryla.smith@utoronto.ca)
Date: Mon Jan 14 2002 - 16:57:26 EST 

Hello All,
I've had a query from one of our facility users regarding the ability of
flow cytometry to detect single FISH probes.  The most recent posting from
the group that I can find is from 1996 and I'm wondering if everyone has
simply decided that flow is not sensitive enough for this.  It was mentioned
that incorporation of digoxigenin into the PCR reaction apparently increased
single to noise ratio, but is it really enough  to enable detection?  Any
input would be greatly appreciated.

Thanks,
Cheryl

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