Happy new year to all of you, One of my users is having a request and I was wondering if some of you could have some advices on mouse dendritic cells staining. I know that we can use the CD208 for human cells but was wondering if there was a more specific marker for mouse. Thanks in advance Nathalie Tessier Cytometrie en flux Institut de recherche cliniques de Montreal Pavel Chrobak wrote: > Dear Nathalie: > We would like to do sorting for mouse spleen and BM derived dendritic > cells, as well as for peritoneal macrophages. Currently we are using Percoll > followed by o/n incubation and then staining with CD11c FITC for DCs and o/n > adherence on plastic followed by staining with Mac1 PE for macrophages. We > were wondering if this is sufficient, or whether we should use different > staining. > Thanks. > Pavel Chrobak > Laboratory of Molecular Biology > email:chrobakp@ircm.qc.ca -- Nathalie Tessier, M.Sc. Adjointe au directeur de l'administration de la recherche Responsable, Service de cytométrie en flux Assistant director, Administration of research Head of flow cytometry service Institut de Recherches Cliniques de Montréal 110, ouest avenue des Pins Montréal, Québec, Canada H1R 1N7 tel:(514) 987-5608 fax:(514) 987-5724
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:59:19 EST