Guinea Pig IF gamma detection

From: Simon Monard (smonard@trudeauinstitute.org)
Date: Wed Jan 09 2002 - 10:41:54 EST

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Hi All, happy new year

I have a friend wishing to detect IF gamma production in guinea pig T-cells. Apparently
there are no commercial antibodies available so he thought of trying to do mRNA detection
in intact cells. I remember trying to do this rather half-heartedly years ago with
horrible background problems. Has anyone had any success doing this? Alternatively does
anyone know of a source of an antibody? He wants a sexy method for a grant proposal,
any other suggestions? I suppose he could extract RNA from sorted cells and do a blot
of some sort, he wanted something more fancy though.

Thanks   Simon Monard

Simon Monard
FACS Lab Manager
Trudeau Institute
Saranac Lake
NY12983

Ph 518 891 3080 X352

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