Contact Cytomation at 1.800.822.9902 (Mountain Time) and ask for Kevin or anyone else in service. Even though I have had a lot of experience with MoFlo, once in awhile They help me remember, in a kind way, something simple I have forgotten about. Don Walker -----Original Message----- From: Xin Zhan [mailto:Xin.Zhan@bmc.org] Sent: Friday, December 14, 2001 7:56 AM To: cyto-inbox Subject: Re: MoFlo operation Dear All; I am a beginner regarding to the operation of MoFlo Cytometer. Could anyone tell me what I could do to solve a problem that I am facing and feeling frustrated about? The problem is that I do not have trigger signals from Flow check after I got my stream straight, ~{!.~}annular ring~{!/~} and beads signal shown through the top pinhole. If I raised the nozzle, I sometimes got a very weak signal from FL1 vs FL2 but FSC vs SSC. When I took off the tube, which connects the universal tower, I saw that laser beam is directed above the hole of the universal tower. Any suggestions? Thanks in advance. Xin Zhan The information contained in this email is intended for the personal and confidential use of the addressee only. It may also be privileged information. If you are not the intended recipient then you are hereby notified that you have received this document in error and that any review, distribution or copying of this document is strictly prohibited. If you have received this communication in error, please notify Celltech Group immediately on: +44 (0)1753 534655, or email 'is@celltech.co.uk' Celltech Group plc 216 Bath Road, Slough, SL1 4EN, Berkshire, UK Registered Office as above. Registered in England No. 2159282
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