RE: MoFlo operation

From: Walker, Don (SEA) (Don.Walker@sea.celltechgroup.com)
Date: Mon Dec 31 2001 - 14:30:30 EST


Contact Cytomation at 1.800.822.9902 (Mountain Time) and ask for Kevin or
anyone else in service. Even though I have had a lot of experience with
MoFlo, once in awhile They help me remember, in a kind way, something simple
I have forgotten about.

Don Walker

-----Original Message-----
From: Xin Zhan [mailto:Xin.Zhan@bmc.org]
Sent: Friday, December 14, 2001 7:56 AM
To: cyto-inbox
Subject: Re: MoFlo operation



Dear All;

I am a beginner regarding to the operation of MoFlo Cytometer.

Could anyone tell me what I could do to solve a problem that I am
facing and feeling frustrated about?

The problem is that I do not have trigger signals from Flow check after
I got my stream straight, ~{!.~}annular ring~{!/~} and beads signal shown
through the top pinhole.  If I raised the nozzle, I sometimes got a
very weak signal from FL1 vs FL2 but FSC vs SSC.  When I took off the
tube, which connects the universal tower, I saw that laser beam is
directed above the hole of the universal tower.

Any suggestions?

Thanks in advance.

Xin Zhan



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