Hello,
We have a client who is trying to sort a fresh water protozoan that
expresses GFP. They are very worried about stressing the cells. They have
found that stressing these cells causes them to release their GFP and thus
show up negative. I was wondering if anybody has determined the minimum
concentration of salt that can be used in the sheath fluid that will still
hold a charge. Presently we use a 1X PBS solution consisting of NaCl, KCl,
Na2HPO4 7H2O, and KH2PO4
Thanx in advance, Marv
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:58:08 EST