Dear colleagues, I'm working In a university teaching hospital, at hematology&oncology ward. We have recently equipped with a bench top flowcytometer(one argone laser) to work mostly on patients with leukemia. I'm fairly familiar with flowcytometry, however I have no experience working on leukemic samples. We have purchased Acute luekemia phenotyping kit from Becton Dickinson company, in which monoclonal antibodies such as CD3,CD19,CD22,CD20,CD5,CD7,CD10,CD13,CD33,HLA-DR, and two vials of negative controls are included. Half of these reagents are conjugated with FITC, and the other half with PE. I'm trying to set up a protocol for acute and chronic leukemia, but I need your help. I know that this kit is not appropriate for chronic leukemia, and I am also not sure the kit that we have now has a good combination for acute leukemia phenotyping. So in this regard, I would like to know the latest antibody combinations for those phenotypings. We can use up to three colors(FITC,PE,PerCP)on our system. I also need to know if I have to work only on BM samples of these patients, or if I can also use their PB cells. Do I have to separate their mononuclear cells? Do I need to run a normal sample every time I run my patients' samples? and if the answer is yes, what good can do this normal sample? I'm assuming that none of the gatings drown on this sample would be used on patient sample. So as you see I have many questions that I'd like to get their answers. I don't know whether it's possible to get a detailed working procedure on those applications that I mentioned above. A protocol that detailes everything from the very beggining to the very end. I'm anxiously looking forward to hear from you and if you don't mind keep in touch with you when I need your consultation. Your valuable time and consideration are highly appreciated. Best Regards, F. Osati __________________________________________________ Do You Yahoo!? Check out Yahoo! Shopping and Yahoo! Auctions for all of your unique holiday gifts! Buy at http://shopping.yahoo.com or bid at http://auctions.yahoo.com
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