Paul . . . thanks for this response. I've tried this, and it works well. I've had some trouble finding a good source for reagent B though. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Flow Core 7416 CCGC 0946 (734) 647-3216, fax (734) 936-7376 kukuru@umich.edu >>> "J.Paul Robinson" <jpr@flowcyt.cyto.purdue.edu> 12/11/01 09:20PM >>> Colleagues: I am going to answer this question in a manner that I hope you all understand (or not)......(think of the bad data issue) You can solve the problem by adding reagent A to reagent B, plotting FL3 Vs FL2. If you add reagent C, then a single histogram of FL5 should do the trick. I think you could also try probe A and probe B, both of which should have the right spectra...Oh, turn the power of laser 1 up a bit, and you should get some valuable data..... sorry, I couldn't resist it... please stop using terms which are totally undefined.... Paul Robinson On 10 Dec 2001, at 11:58, Laura H odges wrote: I'd like to post a question on your website: Can anyone suggest another P-gp accumulation probe other than rhodamine 123 which has too broad of an emission spectrum for my use. I am trying to find a P-gp probe that can be used with other fluorescent markers in channels FL2, FL3, and FL4 in a multicolor assay. __________________________________________________ Do You Yahoo!? Send your FREE holiday greetings online! http://greetings.yahoo.com J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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