Hello, everyone: We're about to undertake a study in horses that will require me to do phenotypic analysis of peripheral blood cells. I have two questions: 1) We want to look at CD3, CD4, CD8, CD21 (or other B cell marker) and CD45. I would like to use monclonals directly conjugated to FITC, PE, etc., but I have not found any so far for equine cells. Do any of you know of a source? 2) We also want to take a look at activated monocytes. Any ideas about how to identify a) the monocytes, and b) whether they are activated? Thanks in advance. Rick Meister * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Richard K. Meister Email: meister.1@osu.edu * * The Ohio State University Voice: (614) 292-9716 * * Dept. of Veterinary Biosciences FAX: (614) 292-6473 * * Cytometry Instrumentation Lab * * 1925 Coffey Road * * Columbus, OH 43210 U.S.A. * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
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