Hi we use rhodamine 123 to measure P-gp activity in conjunction with detecting PE in FL2 and Cy5-PE in FL3. It requires significant compensation but it works. Let me know if you need further info. Best wishes, Rachel ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Laura Hodges > Sent: Monday, December 10, 2001 2:58 PM > To: Cytometry Mailing List > Subject: FACS question > > > I'd like to post a question on your website: > > > Can anyone suggest another P-gp accumulation probe > other than rhodamine 123 which has too broad of an > emission spectrum for my use. I am trying to find a > P-gp probe that can be used with other fluorescent > markers in channels FL2, FL3, and FL4 in a multicolor > assay. > > > __________________________________________________ > Do You Yahoo!? > Send your FREE holiday greetings online! > http://greetings.yahoo.com > >
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