Hi Craig,
I believe there is a way to do this in CellQuest.
First you need to overlay histograms in question -> Plots/Overlay menu.
Then go -> Plots/Histogram Tools and highlight both files then click on "Subtract",
which is in
the form of the button, at the bottom middle position of six buttons in this window
(not very
obvious, I agree). Now, you're automatically going to get save dialog box to save
new subtracted
file with default name e.g. Data.001-Data.002. This is a new FCS file that you can
analyse
separately.
Cheers, Sasha.
--- craig.turner@nbs.nhs.uk wrote:
>
> Hi all, I've come up against an obstacle with the cellquest software from BD. In
fact I'm
> not sure how to do it in any of the software packages I've used. Anyone any bright
ideas?
>
> I want to subtract data from one histogram ("positive") and another ("negative"). Then
> re-analyse the listmode data from this subtracted histogram for other parameters.
>
> Well that's the simplified version anyway. If anyone has done this and can recommend
> away that doesn't involve several weeks of spreadsheets and raw data crunching I'd
> be grateful.
>
> Craig Turner
> NBS UK
>
>
>
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