I am doing research on Flow Cytometers and wonder if anyone can offer
any insight. In particular, I wish to find out about compact flow
cytometers, and flow cytometers using micro fluidic chips. I want to find
out about flow paths investigated including free space, and channel confined
and how sheath flow is handled for each case. We are interested in cell
flow rates (minimum and maximum) and cell types and sizes under
consideration. I am further interested in system architectures,
particularly excitation wavelengths and excitation sources for those
wavelengths, but also geometries, tolerances, and signal to noise ratio
considerations. What fluorescent dyes are used, and what are their
absorption bands? How are micro spheres used in drug discovery with flow
cytometers? Especially what dyes, wavelengths, etc. are involved. I also
want to understand how many and which emission wavelengths are of interest
in flow cytometry for drug discovery. Finally, we are interested in
algorithms that use scattered light- forward and side- to determine cell
size and roughness. What specific angles must be used, how collimated and
coherent does the light need to be, and how are the data analyzed to
determine cell characteristics?
_________________________________________
David Boyett
Electro/Optic Engineer
Axiom Biotechnologies
3550 General Atomics Court
San Diego, CA 92121
858-455-4480
dboyett@axiombio.com <mailto:dboyett@axiombio.com>
www.axiombio.com <http://www.axiombio.com/>
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