I should have noted that the cells are alive at the indo-1 loading and load fine. The problem is happening after the cells are treated with ionomycin, nigericin et al. and placed in the Ca++ buffers. I'm assuming that then the membrane becomes permeable and the indo-1 leaks out. Larry At 09:15 AM 11/28/2001, you wrote: >Larry, > The calibration can't be done with cells that are dead since the indo >1 ester won't be hydrolyzed. We do our calibrations with live cells, get >readings before and after ionomycin (no other permeabilizers) and have no >problems. > Liz Larry W. Arnold, Ph.D. Associate Professor Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:58:05 EST