CD45RA/62L gating and effects of temp/time?

From: Lorrie Epling (lepling@medsfgh.ucsf.edu)
Date: Thu Nov 29 2001 - 09:27:51 EST


We follow HIV positive patients weekly for various subsets, including
CD45RA/CD62L.  Some patients have a spread of CD45RA which varies
week to week (biological variation?).  For many  patients it can be
difficult to distinguish between the negative and positive CD45RA
populations.

In evaluating our panel we have noted a decrease in CD45RA staining
in some samples if the sample is run the next day and also if the
sample is kept in the dark but at a warm room temperature for as
short as 30 minutes.   Any similar experiences with this subset or
suggestions?


Lorrie Epling
Laboratory Supervisor
UCSF/SFGH Core Immunology Lab
General Clinical Research Center
Phone 415-476-5279
Fax 415-206-8200
lepling@medsfgh.ucsf.edu



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