I have a colleague who would like to be able to express a cell surface marker protein in transfected cells that would survive enzymatic dissociation by trypsin and collagenase and be useful for cell analysis and cell sorting by FACS. I wonder if anyone might have any suggestions as to a trypsin-resistant cell surface antigen for which commercial Ab are available? I will certainly appreciate any ideas or pointers that you might be able to share with me. Rick Peppler Research Specialist Flow Cytometry Core Facility Medical University of South Carolina
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