I would like to know if anyone has any experience sorting mouse neurons. I am attempting to sort Hoechst stained cells and have found that these cells tend to want to aggregate very quickly after starting to sort. In fact this aggregation causes abort rate on sorter to exceed 40% even at low flow rates. I am curious to know if there may be something that can be added to sort buffer which consists of PBS,2%FCS,1mM Hepes which will prevent this aggregation during sort. Thanks David David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CNY rm7017 149 13th Street Massachusetts General Hospital-East Charlestown, MA 02129 Tel. (617)726-1683 Fax.(617)724-3164
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