Dear all again, first many thanks about the help with my question about hla typing from a small blood sample. My question is realy related to your response. Esentially it seems very difficult to do a tissue typing on a small blood sample. The work around for me would be, that I do not do the surface staining on fresh blood. However I have to freze or store than the PBMCs, until the tissue lab has done the typing (`7 days). Does anyone know a procedure which allows to freze cells from 300 microl EDTA blood without doing a ficoll gradient ? many thanks Frank Mattes Department of Virology Royal Free Hospital London
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