Hi all, I did cytokine staining with activation and skipped the lyse step, adding perm. solution(BD) directly after surface staining. As I expected - it worked, RBC's were lysed and I got a very clear scatter picture even without gating on CD45 bright cells. Does anyone else do their cytokine assays in whole blood this way? Are there any pros/cons ? I would especially like feedback from any of the BD scientists since they probably have a lot more experience with these reagents. regards, Maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Make a great connection at Yahoo! Personals. http://personals.yahoo.com
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