Dear Linda, Please tell us what flow cytometer(s) you have, then we will be better equipped to offer more advice. On a FACScalibur, we most commonly use FITC/PE/PE-Cy5/APC and can substitute PI or PerCP in FL3. (I believe Cychrome is one of the proprietary names for PE-Cy5) Those combinations are not so easy on our FACStar Plus. You say you cannot use Cychrome and APC together, so you must not have a FACScalibur which can compensate between FL3 and FL4... PE-Tr may work, you would have to get a sample and test it. PE-Cy7 is doubtful, though you just might be lucky enough to have a super red-sensitive detector... Regards, Joseph. At 05:50 19/10/2001, Linda Duncan wrote: >Dear All, >For various reasons, I will be purchasing antibodies from >Caltag, and need to get a combination that will work. I >know that I can use FITC/PE/PerCP/APC but cannot use >FITC/PE/Cychrome/APC because of the overlap in excitation >between Cychrome and APC,but Caltag has three different >fluorochromes on FL3: PE-TR; PE-CY5; PE-CY7. Which FL3 >Fluorochrome from Caltag will work with FITC, PE,and APC? >More importantly which ones won't work. Please help!! >---------------------- >Linda Duncan
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