Dear Flowers, We are currently studying cell cycling in primary murine macrophages by flow cytometry. We have successfully synchronized the cells by serum starvation. Unfortunately, attempts to get these cells to re-enter the cell cycle have been unsuccessful. There have been examples in the literature of cells being induced to re-enter the cell cycle, but these studies were performed with cell lines, not primary cultures. We would be grateful to hear from anyone who has had any luck doing this with primary macrophage cultures. Please send responses to Dr. Maya Kozlowski at Maya_Kozlowski@hc-sc.gc.ca as I will be away from the lab for a while. Thank-you, Gina _____________________________ Gina Graziani-Bowering, PhD Post-Doctoral Fellow Health Canada
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