Ellen, I am using the CyTek AMS sampler for a Calibur cytometer. After some initial technical problems (the device does not handle shipping well) we had good experience with the AMS. It is faster than the BD version, much easier to operate, costs less than half of the BD sampler and you can learn it's operation in a half hour. The disadvantage of the CyTek over the BD sampler is that CyTek had no access to the CellQuest code. To circumvent that the system cleverly operates the CQ acquisition control buttons through a script. Unfortunately you only can specify the total threshold events to be collected. The more popular way of collecting a certain number of events in a gate is not possible with the CyTek AMS. The carryover between wells can be minimized with only a few seconds of backflash and rinse steps. My experience is that increasing the acquisition delay (to allow the pervious sample to run though the sample line) is more useful than prolonged wash times. I would seriously consider the CyTek sampler. Since we work in the same neighborhood you are welcome to bring a plate with samples to Biogen and I'll demonstrate the CyTek machine for you. I (regrettably) have no financial incentive to doing so and try to be objective in my opinion. More sophisticated, faster and robust multiwell samplers are needed. Is anyone listening? Regards, Akos (Biogen) Ellen.Freed@astrazeneca.com on 10/04/2001 03:17:41 PM To: cyto-inbox cc: Subject: 96 well FACS Hi All Has anyone yet used the 96 well format from BD FACScalibur. How is it working? Is 96 well format available from anyone else? Have not kept track of other conversations along this line. Thanks Ellen Freed Ellen Freed Scientist AstraZeneca R&D Boston
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