Dear Qing Li, You are making it very tempting for responses by commercial companies, I know Bob Johnson from Caltag and his products very well and do not expect that he would delivery poor functioning reagents so could it be an incident, storage or shipping issues? Then again if you insist testing an other MPO-antibody you could refer to one of the first antibodies, described in the publication: Characterisation of myeloid leukaemia by monoclonal antibodies, with an emphasis on antibodies against myeloperoxidase, by Ellen van der Schoot, Albert von dem Borne and Pedro Tetteroo; Acta haemat. 78: suppl. 1, 32-40 (1987). Authors are all from Central Laboratory of the Netherlands Red Cross Blood Transfusion Service (CLB) which is also selling these antibodies as "fruits of CLB research" for many years now, see also www.clb.nl <http://www.clb.nl> for more information. Best regards, John John Voorn CLB Reagents Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl> -----Original Message----- From: qli@sbmflab.org [SMTP:qli@sbmflab.org] Sent: Wednesday, October 03, 2001 3:23 PM To: Cytometry Mailing List Subject: MPO/CD22 antibody Hello flowers, We are using Caltag's MPO/CD22 comb antibody for the leukemia phenotyping and get a poor results for MPO. Any suggestion on a good antibody source? Thanks Qing Li, Ph.D. NTTD Department South Bend Medical Foundation South Bend, IN ----------
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