Dr. Shapiro, Thank you for the info concerning the BD UV Laser, as to your point below, it is interesting to note that during training class for the LSR at BD San Jose both issues came up. Several students were perplexed as to why the LSR couldn't cross-beam compensate like the FACSCalibur and it was assumed (perhaps erroneously based on your comment) that 3 lasers made cross-beam compensation undoable or at least not cost-effective. The requirement for Flojo software to perform this compensation off-line seems unwieldy especially as your other point was also commented on by students, that is several of us noticed that the card cage and boards in the LSR appeared identical to those in the Vantage SE sorter, as apparently they are, but when we asked if the LSR was going to go digital as the Vantage SE is none of us could get a straight answer. I for one think BD should consider this option. Gene/UCONN Health If B-D adds the digital processing electronics now available for their sorters to the LSR, it should be feasible to monitor and compensate for He-Cd laser noise, allowing reasonably high resolution DNA/cell cycle analysis. This could also be accomplished with analog circuitry no more complex than they use for cross-beam compensation in the FACSCalibur (and, I believe, the LSR). I use such analog circuitry with my He-Cd lasers, and it has let me operate for a couple of years between retubings with good CV's on DNA analyses.
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