> ---------- > From: qli@sbmflab.org > Sent: Monday, 10 September 2001 23:02 > To: Cytometry Mailing List > Subject: cytoplasmic CD22 > > Quing, > > An easy solution to surface and I/C staining is to first stain surface > with say CD22 FITC, FIX &PERM, then stain with CD22 PE. I would add CD19 > PC5 or similar for B cell confirmation and appropriate controls. > > Greg Hodge > ---------------------------------------------------------------- > Hi, Colleagues, > > I need some help. I am trying to determine the cytoplamic CD22 in > B-Lymphocytes using Caltag's FIX &PERM reagent. Since CD22 also expresses > on the surface of B-Lymphocytes, how to find a method which only measures > cytoplasmic not surface CD22? Also, how can I confirm that I am measuring > cytoplasmic CD22? Thanks. > > Qing Li, Ph.D. > NTTD Department > South Bend Medical Foundation > South Bend, IN > > > > > > ---------- >
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