You could do a "subtraction" method. Stain the same cells with one tube permeabilized and the other tube not permeabilized. The difference is the internal CD22. Randy T. Fischer NIH/NIAMS Building 10, Room 6D57 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: qli@sbmflab.org > Sent: Monday, September 10, 2001 9:32 AM > To: Cytometry Mailing List > Subject: cytoplasmic CD22 > > > Hi, Colleagues, > > I need some help. I am trying to determine the cytoplamic CD22 in > B-Lymphocytes using Caltag's FIX &PERM reagent. Since CD22 also expresses > on the surface of B-Lymphocytes, how to find a method which only measures > cytoplasmic not surface CD22? Also, how can I confirm that I am measuring > cytoplasmic CD22? Thanks. > > Qing Li, Ph.D. > NTTD Department > South Bend Medical Foundation > South Bend, IN > > > > > > ---------- > >
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