Does anyone know of a documented study or has anyone performed a formal study in their lab regarding the use of refrigerated specimens? This would include both CD4 counts and leukemia/lymphoma studies. The problem is that some physicians would like to add on flow to a peripheral blood-edta tube-but it has been refrigerated at 4 degrees C. overnight or for 24 hrs. Also, after preparing a lymph node or tissue specimen the cells remain in 2% FBS/HBSS. The next day more clones may need to be run. In some specimens we find clumping of cells. Does anyone have any experience with this and how was it resolved? The HBSS is without calcium, magnesium, or phenol red. Many thanks for any help.
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