Sorting B-cells/Hybridoma

From: Moss, Delynn M. (dmm3@CDC.GOV)
Date: Wed Aug 29 2001 - 06:54:43 EST

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Hi,

I am new at sorting, and I would be interested in the response from anyone
who has sorted murine B-cells for the production of hybridomas.  My thoughts
are to sort unstained B-cells from stained non B-cells.  Were there benefits
in preparing hybridomas from sorted B-cells compared with preparing
hybridomas from unsorted spleen cells?  If there were benefits, was there a
viability problem with B-cells sorted in sterile PBS?  Were the B-cells kept
cold during the sort?  If you were using a FACStarPLUS, what recovery was
obtained?  How long did it take to sort the cells?

Thanks in advance.

Delynn Moss
CDC
Parasitic Diseases Division

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