Hello everybody, I´d having some problems with a subject: I¹m a user of a FACScalibur device in my Institute, but at the time to try to sorting my neuron cells in this machine, all of the cells go out with a lot of debris and contamination from other users, just like: yeas, bacteria, protozoan, etc. (I do not know exactly what it is). Until this moment I still have not found the way to ensure that my cells are free from that contamination. To clean the internal hoses I had been testing with a 1:10 chlorine solution let it overnight inside the hoses. After that I used DD water (about 1 liter in running mode). And then, just then, put on in my PBS solution to start my flow. But when I put the purified neurons in culture again, invariably become contaminated. Could anybody suggest me some kind of protocol to clean out the Cytometer before I begin to sort??... Specially because I need a lonely neurons whom are very delicate to survive in a culture medium. Thank you very much. Carlos Monter IBT-UNAM Mexico cpmonter@ibt.unam.mx
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