Morning flowers, I've had an enquiry to run some analyses on a sample from an elderly patient with a massive CD4 lymphocytosis. Preliminary molecular work done previously has shown that 94% of purified PBLs are CD4+, and my researcher wants to follow up with some Flow analysis. I've already suggested a CD3/CD4/CD8/CD25, CD4/CD38/CD45RA/CD45RO and CD8/CD38/CD45RA/CD45RO panels. Given the level of expertise on the list I wondered if anyone might care to suggest a 4 colour panel to look at a more tumour specific phenotype. A quick response would be very much appreciated. Thanks Wayne Wayne Turnbull Flow Cytometry Services Peter Gorer Department of Immunobiology 3rd Floor New Guy's House GKT Guy's Hospital London SE1 9RT email: wayne.turnbull@kcl.ac.uk Page: 07666 545524 Tel: 44 (0) 20 7955 4355 Fax: 44 (0) 20 7955 4961
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