Phar Red supplementary question

From: Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Date: Thu Aug 09 2001 - 18:37:53 EST


Hi Nicole Et Al
Can you please clarify "high" vs "low" laser power in this context?

I have one of those OLD BD/Spectra Physics HeNe lasers that you
can't kill with a big stick, still giving about 45mW after all these
years...
I was hoping to use that for APC & APC-Cy7 soon; will it do, or do
we need 100mW that won't last?

Joseph.

At 11:30 9/8/2001, Nicole Baumgarth wrote:
{in relation to another question...}

>As for the general "disappointing" staining with Cy7APC.....can you
>increase laser power? All of the tandem-dyes that I have tried seem to be
>doing increadibly much better at higher laser power. The more the better it
>seems. This is really a limitation for use of the dyes when you try to use
>them with low-power lasers.



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